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Introduction
Transcription of the article from Biomedical Materials, published on June 20, 2020:
Today, scientists are focusing on the development of personalized and tissue-specific bio-inks that can be used in 3D bioprinting technologies.
Platelet-rich plasma is a person-specific source used as a therapeutic complement for the treatment of cartilage injuries because it provides a cocktail of growth factors necessary for wound healing and tissue regeneration.
However, clinical PRP treatments are not satisfactory and need improvement, especially in terms of maintaining bioactivity.
Methods
In this study, we have introduced PRP as a photo-activated and photo-crosslinkable bio-ink for tissue-specific structures for the first time.
We achieved long-term and constant growth factor release while preserving the bioactivity of PRP with satisfactory mechanical properties.
The photo-crosslinked PRP hydrogel was activated by the addition of microwave-induced methacrylated gelatin (Mw-Gel-MA), which is connected to the platelets in PRP through integrin receptors in its structure and chemically crosslinked upon UV irradiation (300-500 nm).
The photoactivation of PRP was carried out using a polychromatic light source in the nearest infrared region (PAC, 600-1200 nm).
Results
The study demonstrated that Gel-MA/PRP hydrogels with desired mechanical properties (low degradation rate and high mechanical strength) released growth factors at a constant rate over the long term through periodic PAC application.
In vitro cell culture studies (viability, proliferation, morphology, histology, immunohistochemistry, biochemistry, gene expression analyses) proved that the proliferation and differentiation of ATDC5 cells increased in the periodically applied Gel MA/PRP hydrogel without any external chemical agents.
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