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kit Microlyzer SVF
The SVF Microlyzer kit provides real isolation of SVF cells as well as real regenerative therapy.
This includes several boxes.
First of all, a collection set (VacLock syringe, 60 cc syringe, Luer collector, etc.), a condensation set for centrifugation (10 cc syringe, Luer caps, etc.), a micronization kit ( Microlyzer 600µm, 1200µm and 2400µm) and lastly a set for mechanical insulation SVF.
This kit therefore enables to create SVF and is handled exclusively by doctors and plastic surgeons.
MC8008
€490.00
Tax excluded
CE Certification
Delivery all over Europe
Free shipping for orders over 500€ HT
The rebirth of the SVF with the Microlyzer Kit :
SVF (Stromal Vascular Faction) is a cell extract that is made in a laboratory.
Most of the time, this fabrication is made from grease, however, the SVF kit offers, through a process of creating adipose tissue, a method completely isolating the SVF cells, excluding both fibrous tissue but also fat cells as well as triglycerides.
SVF is a high concentrate of stem cells being separated from adipose tissue and concentrated. This allows proliferation and multiplication of the latter inside the human body offering a real regenerative treatment that fat does not provide.
An enzyme-free method
The Microlyzer Kit offers a method where the isolation of SVF cells is enzyme-free, unlike fat grafting systems.
Despite the common use of incubating fatty tissue with the enzyme collagenase in order to separate cells from tissues to achieve isolation of SVF, it is very risky to re-inject this enzyme into the human body.
Indeed, if the latter is not completely removed before injection, it can cause considerable damage to the tissue.
This is why the Microlyzer SVF offers a non-enzymatic technique that is fast, rich and risk-free, allowing the isolation of true SVF, all of which leads to a higher regenerative effect.
A high rate of stem cells
This new method provides both regenerative effects with real isolation of SVF, a large number of nucleated cells but also a high rate of stem cells. In addition, it offers fast and cost-effective high viability.
Indeed, it does not generate any enzymatic cost and high investment, as well as no additional components, all allowing a preparation lasting 40 minutes.
Finally, it centrifuges only two times which does not lead to any risk related to enzymes or regulation, offering sterile components and bucket, with a single use liposuction cannula as well as safe injection through a 41 micron filter.
User manual
Harvest set
Liposuction
Attach the liposuction cannula to the VacLok syringe, gradually aspirate until you obtain 60cc of fat.
Transferring the adipose tissue to another syringe
Transfer the adipose tissue collected into the syringe of volume equivalent to 60cc using a luer connector and then close it using a luer cap.
Decantation
Position the fat filled syringe onto the decantation apparatus for 5 minutes in order for the tumescent solution to settle at the bottom.
Getting rid of the tumescent solution
Discard the tumescent solution settlement until you are left with 40cc of fat.
Condensation set
Transferring to 10 cc syringes
Collect 10cc of fat in each syringe so that the initial volume is divided in 4 ways.
Preparing the syringes
Detach the plungers, seal the syringes with the luer caps and prepare them for centrifugation.
Condensing the adipose tissue
Have a sterile member of the surgical team place the sterile bucket on the rotor and then position the 10cc syringes upside down in the bucket (cap at the bottom). Set the spin to 1500G and 8 min, then start the centrifugation process.
Condensed adipose tissue
After centrifugation, three layers become apparent, a tumescent solution and blood at the bottom, condensed fat in the middle and triglyceride (free oil) at the top.
Discard the tumescent solution
Attach the plunger to the syringe and open the cap, get rid of the tumescent solution and blood.
Collecting the condensed fat tissue
Collect only the condensed fat from each 10cc syringe into the single 20cc syringe. The triglyceride (free oil) is stored in the 10cc syringe to be discarded later.
Micronisation kit
2400μm Microlyzer
Fix the 20cc adipose tissue filled syringe onto one side of the 2400µm Microlyzer and fix an empty 20cc syringe onto the other side.
Make a total of 31 passages, one passage being a full push of the condensed tissue through the blade from one side to another until nothing remains in the syringe on which the pressure is applied.
1200μm Microlyzer
Fix the 20cc adipose tissue filled syringe processed through the 2400μm Microlyzer blade onto the 1200μm Microlyzer Blade and fix an empty 20cc syringe onto the other side.
Make a total of 31 passages, one passage being a full push of the condensed tissue through the blade from one side to another until nothing remains in the syringe on which the pressure is applied.
600μm Microlyzer
Fix the 20cc adipose tissue filled syringe processed through the 1200µm Microlyzer blade onto the 600µm Microlyzer Blade and fix an empty 20cc syringe onto the other side.
Make a total of 101 passages, one passage being a full push of the condensed tissue through the blade from one side to another until nothing remains in the syringe on which the pressure is applied.
Keep in mind that the process must be stopped in the syringe opposite to the one on which you made the first pass, hence the odd number of passes.
Mechanical SVF isolation
Transferring the processed adipose tissue to the tubes
Pre-fill the tubes with 2.5cc of saline solution, unfix the adipose tissue filled syringe from the 600µm Microlyzer Blade and evenly split the processed adipose tissue into the two tubes.
Pre SVF isolation
Shake the tube vigorously to mix the saline solution perfectly with the fatty tissue.
Centrifugation
Place the two tubes in the centrifuge and spin at 400G for 10 minutes.
Post centrifugation
After centrifugation, the condensed adipose tissue fractionates into three layers: triglyceride at the top, extracellular matrix remnant in the middle and saline solution at the bottom with the SVF Pellet.
Collecting the SVF
Attach the 18G long needle the 5cc syringe then go to the bottom of the tube by slowly sliding the needle down against the tube’s wall, scrap the SVF cells at the bottom of the tube to mix the SVF and the saline, rotate the tube while looking from the bottom to make sure no pellet are stuck to the bottom, finally collect the saline solution and the SVF. Repeat for the other tube
Filtering with the 41μm Microlyzer
Attach the SVF filled 5cc syringe to the 41µm Microlyzer and an empty 5cc syringe to the other side.
Push the SVF solution through the blade filter into the empty syringe. Unfix the 5cc SVF filled syringe and inject the final solution.
MC8008
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